BIOPHEN H-CoII kit is a chromogenic assay for measuring Heparin
Cofactor II activity in human plasma or in purified systems, using a
chromogenic method, manual or automated, based on the inhibition of a
constant but in excess amount of thrombin.
Heparin Cofactor II is an anticoagulant protein which inhibits specifically
thrombin. This inhibition is highly enhanced by glycoaminoglycans such
as Dermatan Sulfate (1). By contrast with Heparin, Dermatan Sulfate
activates specifically Heparin Cofactor II (7).
The BIOPHEN H-CoII assay is a chromogenic method based on the
inhibition of a constant and in excess amount of thrombin, by the tested
Heparin Cofactor II in presence of dermatan sulfate, and measurement of
residual thrombin by its amidolytic activity on a thrombin specific
chromogenic substrate (SIIa-01). pNA is then released from the substrate.
The amount of pNA released is directly related to the residual thrombin
activity. There is an inverse relationship between the concentration of
Heparin Cofactor II present in the tested sample and color development,
measured at 405 nm
