MEASUREMENT OF PROTEIN Z
MEASUREMENT OF PROTEIN Z
INTENDED USE:
The ZYMUTEST Protein Z kit is a one step immuno-assay for measuring human Protein Z (PZ) in plasma, or in any fluid where PZ can be present.
ASSAY PRINCIPLE:
First, the immunoconjugate, which is a polyclonal antibody specific for PZ coupled to horse radish peroxidase (HRP), is introduced into the microwells coated with a polyclonal antibody specific for PZ. Then, the diluted tested sample is immediately introduced, and the immunological reaction starts. When present, PZ binds onto the polyclonal antibody coated solid phase through one epitope, and fixes the polyclonal antibody coupled to HRP through free epitopes. Following a washing step, the peroxidase substrate, 3,3',5,5' – Tetramethylbenzidine (TMB), in presence of hydrogen peroxide (H2O2), is introduced and a blue colour develops. When the reaction is stopped with Sulfuric Acid, a yellow colour is obtained. The amount of colour developed is directly proportional to the concentration of human PZ in the tested sample.
