Chromogenic anti-Xa method for measuring homogeneously heparin in purified systems or in plasma (tested diluted 1:10) using a two stage method, in compliance with Pharmacopoieas (USP, EP) and FDA guidelines. First, Factor Xa, in excess, is inhibited by heparin in presence of AT III, also in excess (and provided in the assay), then the residual Xa activity is measured by its activity on the specific substrate SXa-11.
|
Assay characteristics
|
Kit components
|
Applications
|
|
Total Assay Time: 5 min.
Detection Threshold: 0.1IU/ml
Dynamic Range: 0 to 2 IU/ml
(0 to 0.2 IU/ml when diluted)
Intra-Assay CV: < 3%
Inter-Assay CV: < 5%
|
R1: 2x2.5 ml Factor Xa
R2: 2x2.5 ml AT III
R3: 2x2.5 ml Factor Xa substrate (SXa-11)
R4: 2x25 ml Sample Diluent
|
Conventional chromogenic inhibitory assay for the measurement of Anti-Xa Heparin (UFH and LMWH) activity in plasma or purified solutions.
|
Streamline laboratory procedures! Click here for ready-to-use reagents and buffers, from Medicago AB. Available in tablets and pouches!
