CHROMOGENIC SUBSTRATES for in vitro research studies if you do not find the substrate you are looking for please contact us at

The BIOPHEN®line of chromogenic substrates is a new line of  high quality chromogenic substrates, which allow testing enzymes involved in coagulation and fibrinolysis.   The BIOPHEN line of substrates are of the highest quality and stability.   The Hyphen Line of Substrates carry all the traditional substrates for Thrombin, Factor Xa, Factor IIa, Kallikrein, Activated Protein C, Plasmin and Plasminogen-SK, and many more.  To find out the chemical composition of the substrate please click on more information on that substrate.

Enzymes are proteins that catalyze most of the chemical reactions that take place in the body. They make it possible for chemical reactions to occur at neutral pH and body temperature. The chemical compound upon which the enzyme exerts its catalytic activity is called a substrate.

Proteolytic enzymes act on their natural substrates, proteins and peptides by hydrolyzing one or more peptide bond(s). This process is usually highly specific in the sense that only peptide bonds adjacent to certain amino acids are cleaved.

Chromogenic substrates are peptides that react with proteolytic enzymes under the formation of color. They are made synthetically and are designed to possess a selectivity similar to that of the natural substrate for the enzyme. Attached to the peptide part of the chromogenic substrate is a chemical group which when released after the enzyme cleavage gives rise to color. The color change can be followed spectrophotometrically and is proportional to the proteolytic activity.

Chromogenic substrate technology was developed in the early 1970s, and has since then become a tool of substantial importance in basic research. The majority of chromogenic substrate applications are found in various clinical fields. In particular they have been used to generate fundamental knowledge of the mechanisms regulating elastase, mast cell tryptase, and many of the factors regulating blood coagulation and fibrinolysis. Furthermore, products based on chromogenic substrate technology have brought a new generation of diagnostics into the clinical laboratory.

These substrates offer a prolonged stability following reconstitution.  We can also offer these substrates in 1g and 5g format. 

Evaluation of chromogenic substrates:  Hyphen BioMed vs Chromogenix 


Code Name Price  
A229061 BIOPHEN CS-61(44) - Urokinase Chromogenic Substrate (1 x 25 mg) $368.00
A229014C Biophen CS-11(65) - Factor Xa Chromogenic Substrate (12x25 mg) $2,663.00