β-D-galactoside galactohydrolase, EC 3.2.1.23
β-galactosidase is a metalloenzyme that splits lactose into glucose and galactose and is coded by a gene (lacZ) in the lac operon of E.Coli. The enzyme β-galactosidase is a commonly used reporter molecule and is a very important marker for the lacZ gene. Because of its high specificity and stability, β-galactosidase is also conjugated to streptavidin, avidin or antibodies to be used as secondary detection reagents in ELISA applications.
The enzyme is a tetramer with a molecular weight of 465 kDa (each subunit 116 000 g/mole). The free thiol groups on the enzyme surface are characterized and are 14-18 mole/mole enzyme. The free thiol groups are useful for derivatisation of the enzyme. The specific activity is 230-375 Units/mg protein at pH 7.0 and 25°C. One unit of enzyme hydrolyses 1 micromole of o-nitrophenyl-beta-D-galactopyronoside per minute. The protein concentration is assayed by the biuret method with BSA as standard.
One vial contains approximately 25 mg (8 kunits) lyophilizate of partly purified enzyme from 1 mM Tris-HCl (pH 7.2), 1 mM MgCl2 and poly(ethylene)glycol 4000 <1.5 mg/ml
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INFORMATION:
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| SKU ID # |
Packaging |
Package Inserts
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MSDS
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| A10-1101-25mg |
25 mg |
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