Granzyme B specific spots produced by 1x10E4 chimpanzee PBMC.
Stimulation: PMA/ionomycin.
Black spots, 2-plate* (*supplied with plates), transparent plates.
The ELISPOT (Enzyme-linked Immunospot) assay has been designed to identify and enumerate individual cells releasing specific proteins in single cell suspensions of lymphoid tissue, CNS tissue, bone marrow or preparations of peripheral blood mononuclear cells (PBMC). The assay is being used increasingly to detect activated T cells and macrophages secreting cytokines, B cells releasing antibodies and activated cytotoxic T cells (CTLs) and NK cells secreting perforin and/or a family of granule-associated serine proteases such as granzyme A and B. The major advantage of the ELISPOT assay is that it detects only activated cells at the single cell level, allowing direct determination of secretory cell frequencies. The high sensitivity and easy performance, without prior in vitro expansion, makes the ELISPOT assay an attractive tool to enumerate cells producing a particular effector molecule during treatment or pathological conditions. The higher sensitivity of ELISPOT in comparison to that of ELISA1 or intracellular staining2 is due to the plate-bound antibodies directly capturing the cytokine released by the cell before it is diluted in the supernatant, trapped by high-affinity receptors or degraded by proteases. The sensitivity of the assay lends itself to measurement of very low frequencies of cytokine-secreting cells (1/300,000).
1) Tanguay, S. and Killion, J.J. 1994. Lymphokine Cytokine Res. 13: 259.
2) Carter, L.L. and Swain, S.L. 1997. Curr. Opin. Immunol. 9: 177.
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INFORMATION: |
| SKU ID # |
Packaging |
Package Inserts |
MSDS |
| ACT169-T2 |
2-plate* black spots |
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