FACTOR XIII ANTIGEN

FACTOR XIII ANTIGEN ELISA

INTENDED USE:

The ZYMUTEST Factor XIII-A kit is a one step "two site" immuno-assay for measuring human Factor XIII (subunit A) (FXIII-A) in plasma, or in any fluid where FXIII can be present.

ASSAY PRINCIPLE:

First, the immunoconjugate, which is a polyclonal antibody specific for FXIII-A coupled to horse radish peroxidase (HRP), is introduced into the microwells coated with a polyclonal antibody specific for FXIII-A. Then, the diluted tested sample is immediately introduced, and the immunological reaction starts. When present, FXIII-A binds onto the polyclonal antibody coated solid phase through one epitope, and fixes the polyclonal antibody coupled to HRP through free epitopes. Following a washing step, the peroxidase substrate, 3,3',5,5' – Tetramethylbenzidine (TMB), in presence of hydrogen peroxide (H2O2), is introduced and a blue colour develops. When the reaction is stopped with Sulfuric Acid, a yellow colour is obtained. The amount of colour developed is directly proportional to the concentration of human FXIII-A in the tested sample.


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