Human IFN-γ / TNF-alpha FluoroSpot kit

For Research Use Only in the United States and Canada
U-CyTech Products are Available Worldwide
Human IFN-γ / TNF-alpha FluoroSpot kit
Code: ACT851-F2
Manufacturer’s Part Number: CT851-F2
Shipping Weight: 2.00 pounds
Quantity in Basket: None
Price: $1,034.00
Quantity:

Product Description

The dual-color cytokine FluoroSpot assay is a modification of the Enzyme-Linked ImmunoSPOT (ELISPOT) assay and is designed to detect double cytokine-secreting T cells at the single cell level. The assay is based on the use of fluorescent- instead of enzymatic-labeled conjugates. Previous attempts to develop an immunoenzymatic dual-color ELISPOT assay were less successful because of problematic interpretation of mixed color spots. The dual-color FluoroSpot assay, however, provides good discrimination between single and double cytokine-secreting cells and is particularly suited to detect T cell subpopulations with a characteristic cytokine profile. The sensitivity of the assay lends itself to measure very low frequencies of cytokine-secreting T cells (1/300,000).

Information

SKU ID# Packaging Website Links
ACT851-F2 2-plate www.cytokine-kits.com
 
Contents Characteristics
Coating antibodies
Biotinylated detector antibodies
Fluorescent-labeled conjugate
Blocking stock solution R
Dilution buffer R
Spot enhancer
Tween-20
PVDF FluoroSpot plates with lids and cover slips
Manual
IFN-γ (green) and TNF-α (red)

Downloads

Instruction Manual MSDS Datasheet Addendeum
Human IFN-γ / TNF-α FluoroSpot kit Human IFN-γ / TNF-α FluoroSpot kit Human IFN-γ / TNF-α FluoroSpot kit Human IFN-γ / TNF-α FluoroSpot kit