IMAC Elution Buffer (50 tablets)
For Research Use Only
Immobilized metal ion affinity chromatography (IMAC) is a widely used separation method for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also some untagged recombinant or native proteins. Medicago's IMAC phosphate and elution buffer tablets have been developed exclusively for use in this method.IMAC buffer tablets are supplied in bottles and in blister packs. One tablet of elution buffer yields a 300 mM sodium chloride, 300 mM imidazole, 50 mM sodium phosphate buffer with pH 8.0 at 22°C.
|SKU ID#||Packaging||Website Links|
|A09-1012-50||50 tablets (100 ml/tablet)||www.buffer-solutions.com|
Dissolve and go.
Exactly pre-weighed tablets in bottles.
Mix elution buffer with phosphate buffer to achieve desired imidazole concentration.
Ultrapure, low UV280 absorbance imidazole.
Formulated from analytical grade chemicals.
|Chemicals: Analytical grade. |
Format: Exactly pre-weighed tablets.
Composition: 300 mM Sodium Chloride, 300 mM Imidazole, 50 mM Sodium Phosphate.
Volume: 100 ml.
pH: 8.0 ± 0.05 at 22°C.
Shelf Life: 3 years after production date.
|Purification of histidine-tagged and non-histidine-tagged recombinant and native proteins using IMAC chromatography. |
The phosphate buffer may be used for other types of affinity chromatography methods.
|Package Inserts||MSDS||Tech Specs||Adaptations|
|IMAC Elution Buffer||N/A||Smart Buffers FAQ||N/A|