Immunosorb A (100 ml)
For Research Use Only
Immunosorb A is a cross-linked agarose matrix with immobilized Protein A. We use recombinant Protein A of high purity.
Protein A binds to specific classes of immunoglobulins and can preferably be used for purification of mouse and rabbit immunoglobulins. The protein binds to the Fc region of immunoglobulins by interacting with the heavy chain. It binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2 and IgG2b. Protein A binds to human IgM, IgA and IgE as well as to mouse IgG3 and IgG1 but with moderate affinity. It does not react with human IgG3 or IgD, nor with mouse IgM, IgA or IgE.
This affinity chromatography matrix is ideal for purifying human, mouse and rabbit immunoglobulins.
Immunosorb A is supplied as a white liquid substrate containing 20% ethanol as preservative. No additional mixing or reagentsare required. Warming the product to room temperature before use is not necessary.
For research use only.
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Pure recombinant Protein A immobilized on a cross-linked agarose matrix.
Strong binding to Fc region of IgG.
High capacity: >20 mg IgG/ml.
|Appearance: White liquid solution. |
Source: Cloned and expressed in E.coli.
Purity: > 95%.
Preservatives: 20% ethanol.
Binding specificity: Protein A binds to specific classes of immunoglobulins.
| Affinity chromatography. |
Purification of immunoglobulins.
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