Human Granzyme B ELISPOT kit

For Research Use Only in the United States and Canada
U-CyTech Products are Available Worldwide
Human Granzyme B ELISPOT kit
Code: ACT229-PB2
Manufacturer’s Part Number: CT229-PB2
Shipping Weight: 2.00 pounds
Quantity in Basket: None
Price: $726.00

Product Description

2-plate black spots (supplied with plates).

The cytokine ELISPOT (Enzyme-Linked ImmunoSPOT) assay is designed to enumerate cytokine-secreting cells in single cell suspensions of lymphoid tissue, central nerve system (CNS) tissue, bone marrow or preparations of peripheral blood mononuclear cells (PBMCs). The assay has the advantage of detecting only activated/memory T cells and has the ability to detect cytokine release in response to antigen by a single cell thereby permitting direct calculation of responder T cell frequencies. The high sensitivity and easy performance, allowing the determination of peptide-reactive T cells without prior in vitro expansion, makes the ELISPOT assay eminently well suited to monitor T cell responses. The higher sensitivity of ELISPOT in comparison to that of ELISA1 or intracellular staining2 is due to the plate-bound antibodies directly capturing the cytokine released by the cell before it is diluted in the supernatant, trapped by high-affinity receptors or degraded by proteases. The sensitivity of the assay lends itself to measurement of very low frequencies of cytokine-secreting cells (1/300,000).

1. Tanguay, S. and Killion, J.J. 1994. Lymphokine Cytokine Res. 13: 259.
2. Carter, L.L. and Swain, S.L. 1997. Curr. Opin. Immunol. 9: 177.

Coating antibodies
Biotinylated detector antibodies
φ-labeled goat anti-biotin antibodies (GABA)
Activator solutions
Blocking stock solution B
Dilution buffer B
PVDF ELISPOT plates with lids and cover slips
Detailed manual


SKU ID# Packaging Website Links
ACT229-PB2 2-plate black spots

Streamline Laboratory Procedures

Ready-to-use reagents and buffers, from Medicago AB. Available in tablets and pouches.


Instruction Manual MSDS Datasheet Addendums
Human Granzyme B ELISPOT kit Human Granzyme B ELISPOT kit Human Granzyme B ELISPOT kit Human Granzyme B ELISPOT kit