AEC coloring system

For Research Use Only in the United States and Canada
U-CyTech Products are Available Worldwide
AEC coloring system
Code: ACT356
Manufacturer’s Part Number: CT356
Shipping Weight: 2.00 pounds
Quantity in Basket: None
Price: $227.00

Product Description

10-plate (plates NOT included). Specificity: Provides the substrate for the ELISPOT assay based on enzymatic staining and performed on PVDF membrane-bottomed plates. The system consists of two items: an AEC (3-amino-9-ethylcarbazole) stock solution and Substrate buffer capsules. Contents: 4 ml AEC stock solution 5 Substrate buffer capsules. Application: The contents of one Substrate buffer capsule should be dissolved in 100 ml 30% ethanol. After complete dissolution, 20 ml of this buffer is thoroughly mixed with 660 µl AEC stock solution. After mixing, the solution should be clear. Once prepared, the solution is stable for 30 minutes at room temperature or for 6 months at ≤ -20°C. Add 100 µl/well in the final step of the U-CyTech ELISPOT assay. Incubate in the dark. Spot development will take place between 15 to 30 minutes and can be monitored by light microscopy. When clear (red) spots have developed, stop the reaction by rinsing both sides of the membrane with demineralised water. Air dry the plate at room temperature. The quality of the spots is preserved for several months when the plate is stored at a dry place in the dark (to prevent bleaching of spots). The product should be used in conjunction with Streptavidin-HRP conjugate (ACT353).


SKU ID# Packaging Website Links
ACT356 10-plate

Streamline Laboratory Procedures

Ready-to-use reagents and buffers, from Medicago AB. Available in tablets and pouches.


Instruction Manaul MSDS Datasheet Adaptations
N/A AEC coloring system AEC coloring system N/A