Human IL-1β ELISA Ab pair
U-CyTech Products are Available Worldwide
20-plate (plates NOT included)
Monoclonal antibody to human interleukin 1 beta (IL-1β): Mouse IgG1
U-CyTech offers matched ELISA antibody pairs for researchers who wish more flexibility and want to usetheir own assay design. These antibodies form the most crucial starting point to perform an ELISA(enzyme-linked immunosorbent assay) for the measurements of cytokine, chemokine and granzyme levelsin biological fluids such as cell culture supernatant, plasma or serum.
Please note that U-CyTech also offer complete ELISA kits, which include all necessities and reagents torun 480 ELISA tests.
The usefulness of sandwich ELISAs in cytokine biology is evident from the many reports published on thissubject. The assay requires two highly specific antibodies (either mono- or polyclonal antibodies) thatbind with high affinity to different epitopes of the analyte of interest. One of the antibodies isimmobilized within the wells of a 96-well microtiter plate. This coating antibody functions to selectivelyimmobilize the analyte from crude protein preparations. The second antibody (detection antibody) islabeled with biotin and binds to another epitope of the analyte. Biotin allows the antibody to interactwith streptavidin molecules. By using HRP (horseradish peroxidase)-labeled streptavidin, the analyte canbe quantified using the enzymatic conversion of an HRP-specific substrate (e.g., TMB).
All antibody pairs, which have been selected by U-CyTech, result in a sensitive ELISA test with a detectionlimit falling within the low picogram range (≤ 5 pg/ml).
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Streamline Laboratory ProceduresReady-to-use reagents and buffers, from Medicago AB. Available in tablets and pouches.
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