Monkey IL-12/23p40 ELISPOT antibody pair

For Research Use Only in the United States and Canada
U-CyTech Products are Available Worldwide
Monkey IL-12/23p40 ELISPOT antibody pair
Code: ACT619-20
Manufacturer’s Part Number: CT619-20
Shipping Weight: 2.00 pounds
Quantity in Basket: None
Price: $1,663.00
Quantity:

Product Description

20-plate (plates NOT included)

Monoclonal antibody to monkey interleukin 12/23p40 (IL-12/23p40) (binds both IL-12 (p35/p40) and IL-23 (p19/p40) heterodimers via the p40 subunit); Mouse IgG1
Biotinylated polyclonal antibody to monkey interleukin 12 (IL-12); Rabbit IgG

The cytokine ELISPOT (Enzyme-Linked ImmunoSPOT) assay is designed to enumerate cytokine-secreting cells in single cell suspensions of lymphoid tissue, central nerve system (CNS) tissue, bone marrow or preparations of peripheral blood mononuclear cells (PBMCs). The assay has the advantage of detecting only activated/memory T cells and has the ability to detect cytokine release in response to antigen by a single cell thereby permitting direct calculation of responder T cell frequencies. The high sensitivity and easy performance, allowing the determination of peptide-reactive T cells without prior in vitro expansion, makes the ELISPOT assay eminently well suited to monitor T cell responses. The higher sensitivity of ELISPOT in comparison to that of ELISA1 or intracellular staining2 is due to the plate-bound antibodies directly capturing the cytokine released by the cell before it is diluted in the supernatant, trapped by high-affinity receptors or degraded by proteases. The sensitivity of the assay lends itself tomeasurement of very low frequencies of cytokine-secreting cells (1/300,000).

1 Tanguay, S. and Killion, J.J. 1994. Lymphokine Cytokine Res. 13: 259.
2 Carter, L.L. and Swain, S.L. 1997. Curr. Opin. Immunol. 9: 177.

Contents:
1 vial coating antibodies (20-plate format each)
1 vial biotinylated detector antibodies (20-plate format each)

Information

SKU ID# Packaging Website Links
ACT619-20 20-plate www.cytokine-kits.com

Streamline Laboratory Procedures

Ready-to-use reagents and buffers, from Medicago AB. Available in tablets and pouches.
 

Downloads

Instruction MSDS Datasheet Addendums
Monkey IL-12/23p40 ELISPOT antibody pair Monkey IL-12/23p40 ELISPOT antibody pair Monkey IL-12/23p40 ELISPOT antibody pair Monkey IL-12/23p40 ELISPOT antibody pair