Human GM-CSF ELISPOT antibody pair

For Research Use Only in the United States and Canada
U-CyTech Products are Available Worldwide
Human GM-CSF ELISPOT antibody pair
Code: ACT638-20
Manufacturer’s Part Number: CT638-20
Shipping Weight: 2.00 pounds
Quantity in Basket: None
Price: $1,663.00
Quantity:

Product Description

20-plate (plates NOT included)

Monoclonal antibody to human granulocyte-macrophage colony stimulating factor (GM-CSF); Mouse IgG1
Biotinylated monoclonal antibody to human granulocyte-macrophage colony stimulating factor (GM-CSF); Rat IgG2a

The cytokine ELISPOT (Enzyme-Linked ImmunoSPOT) assay is designed to enumerate cytokine-secreting cells in single cell suspensions of lymphoid tissue, central nerve system (CNS) tissue, bone marrow or preparations of peripheral blood mononuclear cells (PBMCs). The assay has the advantage of detecting only activated/memory T cells and has the ability to detect cytokine release in response to antigen by a single cell thereby permitting direct calculation of responder T cell frequencies. The high sensitivity and easy performance, allowing the determination of peptide-reactive T cells without prior in vitro expansion, makes the ELISPOT assay eminently well suited to monitor T cell responses. The higher sensitivity of ELISPOT in comparison to that of ELISA1 or intracellular staining2 is due to the plate-bound antibodies directly capturing the cytokine released by the cell before it is diluted in the supernatant, trapped by high-affinity receptors or degraded by proteases. The sensitivity of the assay lends itself to measurement of very low frequencies of cytokine-secreting cells (1/300,000).

1 Tanguay, S. and Killion, J.J. 1994. Lymphokine Cytokine Res. 13: 259.
2 Carter, L.L. and Swain, S.L. 1997. Curr. Opin. Immunol. 9: 177.

Contents:
1 vial coating antibodies (20-plate format each)
1 vial biotinylated detector antibodies (20-plate format each)

Information

SKU ID# Packaging Website Links
ACT638-20 20-plate www.cytokine-kits.com

Streamline Laboratory Procedures

Ready-to-use reagents and buffers, from Medicago AB. Available in tablets and pouches.
 

Downloads

Instruction Manual MSDS Datasheet Addendums
Human GM-CSF ELISPOT antibody pair Human GM-CSF ELISPOT antibody pair Human GM-CSF ELISPOT antibody pair Human GM-CSF ELISPOT antibody pair