XTT Tetrazolium hydroxide - 1200 tests
In Cytotox Products Are Available Worldwide
Colorimetric assay for the quantification of the mitochrondrial metabolism and respiratory chain activity of cells in response to pharmaceutical, chemical and environmental compounds, and nutrients.
Viable cells depend on an intact mitochondrial respiratory chain and an intact mitochondrial membrane. Toxic agents can be identified using mitochondrial dehydrogenases from viable cells.
XTT (2,3-bis[2-methoxy-4-nitro-5-sulfopheny]-2H-tetrazolium-5-carboxyanilide inner salt) is a tetrazolium salt that is cleaved to formazan by the succinate dehydrogenase system which belongs to the mitochondrial respiratory chain, and is only active in viable cells. The mitochondrial succinate dehydrogenase reduces the yellow tetrazolium salt into soluble orange formazan in the presence of an electron coupling reagent.
In contrast to the insoluble formazan salt crystals of MTT, XTT is converted to a water-soluble formazan product without the need for a solubilization step prior spectrophotometric quantification. The enzyme activity is measured at 480 nm (optimum) or at 450 nm.
|SKU ID#||Packaging||Storage Temp||Website Links|
|AKXT96-1200||1200 tests without microplates||www.cytoxicity-test.com|
Streamline Laboratory ProceduresReady-to-use reagents and buffers, from Medicago AB. Available in tablets and pouches.
|Instructions For Use||Calculation||MSDS||Additional Info|