XTT Tetrazolium hydroxide - 1200 tests

For Research Use Only in the United States and Canada
In Cytotox Products Are Available Worldwide
XTT Tetrazolium hydroxide - 1200 tests
Code: AKXT96-1200
Manufacturer’s Part Number: KXT96-1200
Quantity in Basket: None
Price: $360.00
Quantity:

Product Description

Colorimetric assay for the quantification of the mitochrondrial metabolism and respiratory chain activity of cells in response to pharmaceutical, chemical and environmental compounds, and nutrients.

Viable cells depend on an intact mitochondrial respiratory chain and an intact mitochondrial membrane. Toxic agents can be identified using mitochondrial dehydrogenases from viable cells.

XTT (2,3-bis[2-methoxy-4-nitro-5-sulfopheny]-2H-tetrazolium-5-carboxyanilide inner salt) is a tetrazolium salt that is cleaved to formazan by the succinate dehydrogenase system which belongs to the mitochondrial respiratory chain, and is only active in viable cells. The mitochondrial succinate dehydrogenase reduces the yellow tetrazolium salt into soluble orange formazan in the presence of an electron coupling reagent.

In contrast to the insoluble formazan salt crystals of MTT, XTT is converted to a water-soluble formazan product without the need for a solubilization step prior spectrophotometric quantification. The enzyme activity is measured at 480 nm (optimum) or at 450 nm.

Information

SKU ID# Packaging Storage Temp Website Links
AKXT96-1200 1200 tests without microplates www.cytoxicity-test.com

Streamline Laboratory Procedures

Ready-to-use reagents and buffers, from Medicago AB. Available in tablets and pouches.

Downloads

Instructions For Use Calculation MSDS Additional Info
XTTN/AXTT XTT