Clotting method

HEMOCLOT Thrombin Inhibitors
For Research Use Only in the United States and Canada

Clotting, Deficient Plasmas, & Chromogenic Assays products are available worldwide

510K Filing In Progress

Assay designed for the quantitative measurement of Hirudin, Agatroban, Melagatran, Ximelagatran, and other direct Thrombin Inhibitors in plasma; contains highly purified human thrombin (same quality as HEMOCLOT Thrombin), and an optimized human pooled plasma, used as substrate. The assay allows specific measurement of Hirudin or other Thrombin inhibitors by their direct antithrombin activity. It is insensitive to the Prothrombin and fibrinogen concentrations in the tested patient plasma specimen. According to the posology used, and to the patient's clinical context (antithrombin therapy, substitutive anticoagulant in presence of HIT or extra-corporeal circulation), the assay works with different plasma dilutions. Assay dynamic ranges are usually from 0 to 2 µg/mL Hirudin (low range) or from 0 to 5 µg/mL (high range).

Assay characteristics

Kit components

Applications

Total Assay Time:                                                < 3 min.

Detection Threshold:                                        < 0.1 µg/mL

Dynamic Range:                                             0 - 5 µg/mL

Intra-Assay CV:                                                        < 4%

Inter-Assay CV:                                                        < 6%

R1: 3x1 mL normal pooled citrated plasma

R2: 3x1 mL highly purified human calcium thrombin (a form)

for the quantitative measurement of any direct thrombin inhibitor drug such as Hirudin, Argatroban, Melagatran, Ximelagatran