PAC Acid Phosphatase
For Research Use Only in the United States and Canada
IN CYTOTOX products are available worldwide
PAC test principle
The acid phosphatase (PAC) is a hydrolase with a pH optimum below 7 and catalyzes the dephosphorylation of organic ortho-phosphoric esters. The enzyme has transferase activity leading to the fixation of a phosphate to the hydroxyl group of an alcohol. PAC is located in the golgi apparatus and therefore a marker for lysosomal activity.
The membrane associated PAC cleaves the colorless substrate p-nitrophenyl phosphate to the colorless pnitrophenol which is measured spectrophotometrically at 405 nm after conversion to the yellow p-nitrophenolate form by addition of 1 M NaOH.An increase or decrease in cell numbers results in a concomitant increase or decrease in the amount of substrate converted by the test compound.
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| INFORMATION |
| SKU ID # |
Packaging |
Package Inserts |
MSDS |
Adaptations |
Price |
| AKPA 96.300 |
300 tests without microplates |
|
|
|
$235.00 |
|
| AKPA 96.310 |
300 tests with 4 microplates, 4 reservoirs |
|
|
|
$335.00 |
|
| AKPA 96.1200 |
1200 tests without microplates |
|
|
|
$500.00 |
|
| AKPA 96.1210 |
1200 tests with 16 microplates, 4 reservoirs |
|
|
|
$850.00 |
|
|
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