LDHe Extracellular Lactate Dehydrogenase (1200 tests)
- Extracellular LDH test principle: Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Lactate dehydrogenase (LDH) is a stable cytoplasmic enzyme present in all cells, and is rapidly released into the cell culture supernatant upon membrane damage or cell lysis. This assay is a fast and simple method to determine changes in the plasma membrane upon incubation with a test compound LDH activity reduces pyruvate to lactate by oxidizing NADH to NAD+. NADH degradation is measured spectrophotometrically by a decrease in the OD at 340 nm.
- Reconsitituion Solution 2 x 180 mL.
- NADH 2 x 130 mL (after reconstitution).
- Pyruvate 1 x 60 mL (after reconstitution).
- 1 Instruction manual.
Colorimetric assay for the quantification of membrane integrity and cellular viability in response to pharmaceutical, chemical and environmental compounds, and nutrients.
Material Safety Data Sheet (MSDS)
Certificate of Analysis (COA)