Immobiliseeritud metallioonide afiinsuskromatograafia (IMAC) elueerimispuhver (50 tabletti)
Töötlemine...

Immobiliseeritud metallioonide afiinsuskromatograafia (IMAC) elueerimispuhver (50 tabletti)

Code: A09-1012-50
Manufacturer’s Part Number: 09-1012-50
Product size: 50 tablets
Kõrvaldamine: RUO

Price: $258.00

Kogus

Toote kirjeldus

Medicago SmartBuffers® pre-formulated tablet — dissolves in specified volume of deionized water to prepare IMAC imidazole elution buffer. GMP manufactured by Medicago AB (Uppsala, Sweden). For research use. Available worldwide.

SmartBuffers® eliminate the need to weigh individual buffer salts — each pre-measured tablet dissolves in the specified volume to deliver precise pH, molarity, and ionic strength. Reduces preparation time, eliminates weighing error, and ensures reproducible buffer composition batch-to-batch. For elution of His-tagged recombinant proteins from IMAC (nickel or cobalt) affinity columns during protein purification.

  • Pre-weighed tablet format — no weighing errors, instant preparation
  • GMP manufactured — lot-to-lot consistency
  • Imidazole-based elution — competes with histidine tags for metal ion binding
  • For Ni-NTA, Co²⁺, and other IMAC resins
  • Available in 10 and 50 tablet pack sizes

Toote omadused

  • Dissolve and go.
  • Exactly pre-weighed tablets in bottles.
  • Mix elution buffer with phosphate buffer to achieve desired imidazole concentration.
  • Ultrapure, low UV280 absorbance imidazole.
  • Formulated from analytical grade chemicals.

Toote komponendid

  • Chemicals: Analytical grade.
  • Format: Exactly pre-weighed tablets.
  • Composition: 300 mM Sodium Chloride, 300 mM Imidazole, 50 mM Sodium Phosphate.
  • Volume: 100 mL.
  • pH: 8.0 ± 0.05 at 22°C.
  • Shelf Life: 3 years after production date.

Toote kasutusvaldkonnad

Purification of histidine-tagged and non-histidine-tagged recombinant and native proteins using IMAC chromatography.

The phosphate buffer may be used for other types of affinity chromatography methods.