Ames — Semisolid S. typhimurium TA1535[pSK1002] for umuC SOS Assay

Только для научных исследований. Не предназначено для использования в диагностических процедурах.

Salmonella typhimurium TA1535[pSK1002] tester strain, semisolid format. 250 µL. Store at −70°C.

TA1535[pSK1002] combines base-pair substitution detection (TA1535 histidine mutation) with SOS genotoxicity reporting via the umuC::lacZ fusion on the pSK1002 plasmid. Upon exposure to genotoxic chemicals, the umuC gene is induced as part of the SOS DNA damage response. The pSK1002 plasmid carries a umuC–lacZ gene fusion; SOS induction is quantified by measuring β-galactosidase activity via conversion of ONPG (o-nitrophenyl-β-D-galactopyranoside) to yellow o-nitrophenol at 420 nm.

• Basis of the umuC SOS genotoxicity assay (ISO 13829)
• Detects clastogenic and base-pair substitution genotoxins via SOS induction
• Colorimetric β-galactosidase readout at 420 nm
• For umuC Easy CS kit and TG Medium (AUMU-TGM)
• Validated for water and environmental chemical genotoxicity screening