Immobilized Metal Ion Affinity Chromatography (IMAC) Elution Buffer (10 tablets)
Medicago AB Products Are Available Worldwide
For Research Use Only
Immobilized Metal ion Affinity Chromatography (IMAC) is a widely used separation method for purifying proteins and peptidesthat have an affinity for metal ions, such as histidine-tagged proteins but also some untagged recombinant or native proteins. Medicago's IMAC phosphate and elution buffer tablets have been developed exclusively for use in this method.
IMAC buffer tablets are supplied in bottles and in blister packs. One tablet of elution buffer yields a 300 mM sodium chloride, 300 mM imidazole, 50 mM sodium phosphate buffer with pH 8.0 at 22°C.
Purification of histidine-tagged and non-histidine-tagged recombinant and native proteins using IMAC chromatography.
The phosphate buffer may be used for other types of affinity chromatography methods.
Instructions For Use