The Ames MPF and Ames II assays are modified versions of the Ames test using liquid culture instead of agar plates. The assays are based on the fluctuation method cited in OECD Guidline 471 but use 384-well microtiter plates and have a colorimetric read-out. The great advantage of this format is the lower amount of sample needed and much easier handling. The Ames MPF assays use TA98 and/or TA100, TA1535, TA1537. All these test strains are recommended in the current, formal test guidelines.
The Ames II assay differs from the Ames MPF assays in that it comprises TA98 and TAMix, consisting of 6 histidine mutant Salmonella tester strains, TA7001-TA7006. Each of these mutants can be reverted only by a specific transition or transversion, so that all possible base-pair changes can be detected by TAMix. The TA700x tester strains are not included among the recommended tester strains in the current, formal test guidelines.
Xenometrix offers practical training and in-house testing of the Ames MPF and Ames II assays in its facilities in Allschwil.
| Reference | Product Name | Price | ||
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AA01-210-S2-P
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Ames MPF 98/100 - with S9 and Positive Controls | $874.00 | |
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AA10-210
|
Ames MPF 98/100 | $2,886.00 | |
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AA10-210-S2-P
|
Ames MPF 98/100 - with S9 and Positive Controls | $2,583.00 | |
|
|
AJ05-210
|
Ames MPF 98/100 Aqua | Price Inquiry | |
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APCO-0800
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S9 Cofactor Kit for Ames MPF | $233.00 | |
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APME-EM22
|
Ames MPF E.coli Exposure Medium | $68.00 | |
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APME-IM31
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Ames MPF E.coli Reversion Indicator Medium | $209.00 | |
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APMM-EM02
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Ames MPF / Ames II Salmonella Exposure Medium | $65.00 | |
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APMM-GM00
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Ames MPF/Ames II Growth Medium | $65.00 | |
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APMM-IM10
|
Ames MPF/Ames II Salmonella Reversion Indicator Medium | $200.00 | |
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APPC-AA01
|
Aminoanthracene - Positive Controls for Ames MPF, umuC (100 micrograms) | $64.00 | |
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APPC-AC02
|
N4-Aminocytidine - Positive Control for Ames MPF, umuC | $117.00 | |
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APPC-AF10
|
2-Aminofluorene - Positive Controls for Ames MPF, Ames II, umuC | $141.00 | |
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APPC-AR05
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Aminoacridine - Positive Controls for Ames MPF, umuC (1000 micrograms) | $90.00 | |
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APPC-NF00
|
Nitrofluorene - Positive Controls for Ames MPF, umuC (20 micrograms) | $70.00 | |
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APPC-NQ02
|
Nitroquinoline-N-oxide - Positive Controls for Ames MPF, umuC (20 micrograms) | $89.00 | |
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APRS-PB01
|
Ames Rat Liver S9 (Lyophilized), PB/BN Induced (1 mL) | $99.00 | |
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APRS-PB02
|
Ames Rat Liver S9 (Lyophilized), PB/BN Induced, (2 mL) | $128.00 | |
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APSS-0110
|
Ames - Semisolid S. Typhimurium Strains, TA98 | $145.00 | |
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APSS-0111
|
Ames-Semisolid S. Typhimurium Strains, TA100 | $145.00 |