EC-Blue Enhanced Substrate (TMB) (250 mL)
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EC-Blue Enhanced Substrate (TMB) (250 mL)

Code: A10-9405-250
Manufacturer’s Part Number: 10-9405-250
Product size: 250 mL

Price: $121.00

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Product Description

Medicago AB Products Are Available Worldwide

For Research Use Only

EC-Blue Enhanced Substrate (TMB) Substrate is a ready-to-use liquid substrate for HRP, specially developed for ELISA applications. TMB Substrate turns deep blue (620-650 nm) in the presence of a peroxidase-labelled conjugate. Color development can be stopped by adding 0.5 M H2SO4 (A10-9500-50), which turns the substrate yellow (450 nm).

TMB contains a proprietary ready-to-use chromogen substrate mixture. The solution has a straw-yellow color at room temperature and a light greenish tint when stored at 4°C. The color returns to normal when warmed to room temperature.

For laboratory use only.

Product Characteristics

  • Ready-to-use liquid substrate.
  • Substrate for assays using horseradish peroxidase (HRP).
  • Low background.
  • High sensitivity.
  • Long stability.

Product Components

  • Appearance: Straw yellow color at room temperature. Slightly green tint when stored at +4°C. Color returns to yellow when stored at room temperature.
  • Protein content: One bottle of EC Blue consist of a solution containing chromogen substrate reagents already mixed, pH 3, 8.
  • Functional characteristics: Functional testing shows less than 10% average standard deviation from previously approved lots of chromogen. Test performed in the same assay. Background optical densities shows less than 10% deviation from that of previously approved lots when tested in the same assay.
  • Storage: 2°C to 8°C.

Product Applications

Recommended for ELISA procedures.

Material Safety Data Sheet (MSDS)

Technical Data

Certificate of Analysis (COA)

Other Documents