Assay designed for the quantitative measurement of Hirudin and other direct Thrombin Inhibitors in plasma; contains highly purified human thrombin (same quality as HEMOCLOT Thrombin), and an optimized human pooled plasma, used as substrate. The assay allows specific measurement of Hirudin or other Thrombin inhibitors by their direct antithrombin activity. It is insensitive to the Prothrombin and fibrinogen concentrations in the tested patient plasma specimen. According to the posology used, and to the patient's clinical context (antithrombin therapy, substitutive anticoagulant in presence of HIT or extra-corporeal circulation), the assay works with different plasma dilutions. Assay dynamic ranges are usually from 0 to 2 µg/mL (low range) or from 0 to 5 µg/mL (high range), when used for hirudin testing.
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Assay characteristics
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Kit components
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Applications
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Total Assay Time: < 3 min.
Detection Threshold: < 0.1 µg/mL
Dynamic Range: 0 - 5 µg/mL
Intra-Assay CV: < 4%
Inter-Assay CV: < 6%
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3x25 ml (ACK002L)
R1: Normal pooled citrrated plasma
R2: Highly purified human calcium thrombin (α form)
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Quantitative measurement of Hirudin, and anti-Thrombin activities in plasma (eg Argatroban® Plasma Calibrator (#ASC030K), Argatroban® Control Plasma (#ASC035K)). |
Download Technical File for HEMOCLOT Thrombin Inhibitors
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