MUTAGENICITY - AMES II & AMES MPF

The Ames MPF and Ames II assays are modified versions of the Ames test using liquid culture instead of agar plates. The assays are based on the fluctuation method cited in OECD Guidline 471 but use 384-well microtiter plates and have a colorimetric read-out. The great advantage of this format is the lower amount of sample needed and much easier handling. The Ames MPF assays use TA98 and/or TA100, TA1535, TA1537. All these test strains are recommended in the current, formal test guidelines.

The Ames II assay differs from the Ames MPF assays in that it comprises TA98 and TAMix, consisting of 6 histidine mutant Salmonella tester strains, TA7001-TA7006. Each of these mutants can be reverted only by a specific transition or transversion, so that all possible base-pair changes can be detected by TAMix. The TA700x
tester strains are not included among the recommended tester strains in the current, formal test guidelines.

Xenometrix offers practical training and in-house testing of the Ames MPF and Ames II assays in its facilities in Allschwil.


Code Name Price  
APSS-0113 Ames - Semisolid S. typhimurium Strains - TA1537 $152.00
APSS-0115 Ames - Semisolid E.coli Strains - E.coli uvrA $152.00
APSS-0116 Ames - Semisolid E.coli Strains - E.coli pKM101 $152.00
APME-EM22 Ames MPF E.coli Exposure Medium, 50 ml $75.00
APME-IM31 Ames MPF E.coli Reversion Indicator Medium, 550 ml $328.00
APMM-EM02 Ames MPF Salmonella Exposure Medium, 50 ml $75.00
APMM-GM00 Ames MPF Growth medium, 50 ml $75.00
APMM-IM10 Ames MPF Salmonella Reversion Indicator Medium, 550 ml $328.00
APPC-AA01 2-Aminoanthracene - Positive Controls for Ames MPF, umuC $42.00
APPC-AA02 2-Aminoanthracene - Positive Controls for Ames MPF, umuC $128.75
APPC-AR05 9-Aminoacridine - Positive Controls for Ames MPF, umuC $59.00
APPC-NF00 2-Nitrofluorene - Positive Controls for Ames MPF, umuC $42.00
APPC-NQ02 4-Nitroquinoline-N-oxide - Positive Controls for Ames MPF, umuC $42.00
APRS-AC00 AMES RAT LIVER S9 (Lyophilized), 1254 Aroclor induced, 0.4 ml $72.00
APRS-AC01 AMES RAT LIVER S9 (Lyophilized), 1254 Aroclor induced, 1 ml $105.00
APRS-AC02 AMES RAT LIVER S9 (Lyophilized), 1254 Aroclor induced, 2 ml $135.00
APRS-PB00 AMES RAT LIVER S9 (Lyophilized), PB/BN Induced, 0.4 ml $79.50
APRS-PB01 Ames Rat Liver S9 (Lyophilized), PB/BN Induced, 1 ml $111.00
APRS-PB02 Ames Rat Liver S9 (Lyophilized), PB/BN Induced, 2 ml $145.00
APCO-0800 S9 Cofactor Kit for Ames MPF $169.00