Narcissus pseudonarcissus lectin (NPA/NPL) (50 mg)
For Research Use Only
Narcissus pseudonarcissus lectin (NPL) is isolated from daffodils. It exists as a dimeric protein (molecular weight 26 kDa) composed of two subunits of 13 kDa each. NPL dissociates into its monomers below pH 5.0 and above pH 9.0.
NPL is mitogenic for human lymphocytes. Specificity for blood group: non-reactive with human erythrocytes.
Narcissus pseudonarcissus lectin is supplied as a white lyophilized powder in 10 mM CH3COONH4.For laboratory use only.
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Sugar specificity: α-D-mannose.
Non-reactive with human erythrocytes.
|Appearance: White lyophilized powder or flocculate. |
Molecular weight: 26 kDa.
Sugar specificity: D-GalNAc.
Activity: Agglutinates rabbit erythrocytes at <0.1 µg/ml, inhibited by 10 mM D-galactose. NPL agglutinates both normal and trypsine treated rabbit erythrocytes.
Microorganisms: < 100 CFU/g.
Protein content: > 90%, OD280nm, Abs 280 nm (ε 1.35 = 1 mg/mL).
Identity: SDS-PAGE, one band at 13 kDa.
Shelf life: > Five years when stored at -20°.
|Mitogenic studies of human lymphocytes.|
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