GABA conjugate; ELISPOT

For Research Use Only in the United States and Canada
U-CyTech Products are Available Worldwide
GABA conjugate; ELISPOT
Code: ACT351
Manufacturer’s Part Number: CT351
Shipping Weight: 2.00 pounds
Quantity in Basket: None
Price: $138.00
Quantity:

Product Description

5-plate (plates NOT included). Specificity: GABA conjugate forms part of the silver-staining system for the U-CyTech ELISPOT assay performed on either polystyrene or PVDF membrane-bottomed plates. Contents: Lyophilized product; sufficient for 480 ELISPOT determinations. Buffer prior to lyophilization: 0.5 ml PBS + 125 mM trehalose + 1% BSA. Preservative free. Application: Reconstitute the lyophilized contents by injecting 500 µl of sterile distilled water into the vial. Mix the solution gently for approximately 15 seconds and allow it to stand for 2 minutes at room temperature. Avoid vigorous shaking. Dilute the reconstituted GABA 50x in Dilution buffer (10x diluted from stock in PBS). Apply 50 µl/well. Cover the plate and incubate 1 hour at 37°C, 100% humidity. The product should be diluted in either Dilution buffer T (ACT358; when using polystyrene-bottomed plates) or in Dilution buffer B (ACT364; PVDF membrane bottomed plates) and it should be used in conjunction with substrate solutions Activator I and II (ACT355).

Information

SKU ID# Packaging Website Links
ACT351 5-plate www.cytokine-kits.com

Streamline Laboratory Procedures

Ready-to-use reagents and buffers, from Medicago AB. Available in tablets and pouches.
 

Downloads

Instruction Manual MSDS Datasheet Adaptations
N/A GABA conjugate; ELISPOT GABA conjugate; ELISPOT N/A