ICE peptide pool; ELISPOT control
U-CyTech Products are Available Worldwide
50 tests (plates NOT included).
Specificity: To be used as an antigen-specific positive control in the human IFN-γ ELISPOT assay. The pool consist of 23 peptides of Influenza A virus (Flu), Cytomegalovirus (CMV) and Epstein Barr virus (EBV) epitopes which are recognized by CD8+T cells and presented by 11 class I HLA-A and HLA-b alleles prevalent among Caucasian individuals. (Reference: Currier, J.R. et al. 2002. J. Immunol. Methods 260: 157-172). Application: Reconstitute the lyophilized contents by injecting 250 µl of sterile distilled water into the vial. Mix the solution gently for approximately 15 seconds and allow it to stand for 2 minutes at room temperature. Avoid vigorous shaking. Working concentration: 20x in culture medium containing human PBMC. Preferred cell/peptide volume: 100 µl well. Contents: Lyophilized ICE peptide pool. 23 x 5 µg peptide/vial. Buffer prior to lyophilization: 0.25 ml PBS + 125 mM trehalose + 1% BSA. Preservative free.