ICE peptide pool; ELISPOT control

For Research Use Only in the United States and Canada
U-CyTech Products are Available Worldwide
ICE peptide pool; ELISPOT control
Code: ACT370
Manufacturer’s Part Number: CT370
Shipping Weight: 2.00 pounds
Quantity in Basket: None
Price: $115.00
Quantity:

Product Description

50 tests (plates NOT included).

Specificity: To be used as an antigen-specific positive control in the human IFN-γ ELISPOT assay. The pool consist of 23 peptides of Influenza A virus (Flu), Cytomegalovirus (CMV) and Epstein Barr virus (EBV) epitopes which are recognized by CD8+T cells and presented by 11 class I HLA-A and HLA-b alleles prevalent among Caucasian individuals. (Reference: Currier, J.R. et al. 2002. J. Immunol. Methods 260: 157-172). Application: Reconstitute the lyophilized contents by injecting 250 µl of sterile distilled water into the vial. Mix the solution gently for approximately 15 seconds and allow it to stand for 2 minutes at room temperature. Avoid vigorous shaking. Working concentration: 20x in culture medium containing human PBMC. Preferred cell/peptide volume: 100 µl well. Contents: Lyophilized ICE peptide pool. 23 x 5 µg peptide/vial. Buffer prior to lyophilization: 0.25 ml PBS + 125 mM trehalose + 1% BSA. Preservative free.

Information

SKU ID# Packaging Website Links
ACT370 50 tests www.cytokine-kits.com

Streamline Laboratory Procedures

Ready-to-use reagents and buffers, from Medicago AB. Available in tablets and pouches.
 

Downloads

Instruction Manual MSDS Datasheet Adaptations
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