Anti-CD3/CD28 stimulus for human PBMC

For Research Use Only in the United States and Canada
U-CyTech Products are Available Worldwide
Anti-CD3/CD28 stimulus for human PBMC
Code: ACT372
Manufacturer’s Part Number: CT372
Shipping Weight: 2.00 pounds
Quantity in Basket: None
Price: $181.00
Quantity:

Product Description

10 ml (50 tests per vial)

Specificity: To be used as a polyclonal T-cell activator in the human cytokine ELISPOT analyses. The vial contains two antibodies specific for human CD3 and CD28. A variety of different cytokines are elicited by this antibody combination including IFN-gamma, IL-2, IL-4, IL-5, IL-10 and IL-13. Application: Reconstitute the lyophilized contents by injecting 500 µl of sterile distilled water into the vial. Mix the solution gently for approximately 15 seconds and allow it to stand for 2 minutes at room temperature. Avoid vigorous shaking. Working concentration: 100x in culture medium containing ≥ 104 human PBMC/ml. Add 10 µl of the stock solution to 990 µl culture medium containing ≥ 104 human PBMC/ml (50 tests per vial). Contents: Optimal amount of both CD3 and CD28 monoclonal antibodies for ELISPOT analyses. Buffer prior to lyophilization: 0.5 ml PBS + 125 mM trehalose + 1% BSA. Preservative free.

Information

SKU ID# Packaging Website Links
ACT372 10 ml www.cytokine-kits.com

Streamline Laboratory Procedures

Ready-to-use reagents and buffers, from Medicago AB. Available in tablets and pouches.
 

Downloads

Instruction Manual MSDS Datasheet Adaptations
N/A Anti-CD3/CD28 stimulus for human PBMC Anti-CD3/CD28 stimulus for human PBMC N/A