Mouse IL-10 ELISA Antibody Pair
U-CyTech Products are Available Worldwid
10-plate format (plates NOT included)
Monoclonal antibody to mouse interleukin 10 (IL-10); Rat IgM
The usefulness of sandwich ELISAs in cytokine biology is evident from the many reports published on this subject. The assay requires two highly specific antibodies (either mono- or polyclonal antibody) that bind with high affinity to different epitopes of the analyte of interest.One of the antibodies is immobilized within the wells of a 96-well microtiter plate. The coating antibody functions to selectively immobilize the analyte from crude protein preparations. The second antibody (detection antibody) is labeled with biotin and binds to another epitope of the analyte. Biotin allows the antibody to interact with streptavidin molecules.By using HRP (horseradish peroxidase)-labeled streptavidin, the analyte can be quantified using the enzymatic conversion of an HRP-specific substrate (e.g., TMB).
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Streamline Laboratory ProceduresReady-to-use reagents and buffers, from Medicago AB. Available in tablets and pouches.
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