Immobilized Metal Ion Affinity Chromatography (IMAC) Elution Buffer (50 tablets)
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Immobilized Metal Ion Affinity Chromatography (IMAC) Elution Buffer (50 tablets)

Code: A09-1012-50
Manufacturer’s Part Number: 09-1012-50
Product size: 50 tablets
Clearance: RUO

Price: $258.00

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Product Description

Medicago SmartBuffers® pre-formulated tablet — dissolves in specified volume of deionized water to prepare IMAC imidazole elution buffer. GMP manufactured by Medicago AB (Uppsala, Sweden). For research use. Available worldwide.

SmartBuffers® eliminate the need to weigh individual buffer salts — each pre-measured tablet dissolves in the specified volume to deliver precise pH, molarity, and ionic strength. Reduces preparation time, eliminates weighing error, and ensures reproducible buffer composition batch-to-batch. For elution of His-tagged recombinant proteins from IMAC (nickel or cobalt) affinity columns during protein purification.

  • Pre-weighed tablet format — no weighing errors, instant preparation
  • GMP manufactured — lot-to-lot consistency
  • Imidazole-based elution — competes with histidine tags for metal ion binding
  • For Ni-NTA, Co²⁺, and other IMAC resins
  • Available in 10 and 50 tablet pack sizes

Product Characteristics

  • Dissolve and go.
  • Exactly pre-weighed tablets in bottles.
  • Mix elution buffer with phosphate buffer to achieve desired imidazole concentration.
  • Ultrapure, low UV280 absorbance imidazole.
  • Formulated from analytical grade chemicals.

Product Components

  • Chemicals: Analytical grade.
  • Format: Exactly pre-weighed tablets.
  • Composition: 300 mM Sodium Chloride, 300 mM Imidazole, 50 mM Sodium Phosphate.
  • Volume: 100 mL.
  • pH: 8.0 ± 0.05 at 22°C.
  • Shelf Life: 3 years after production date.

Product Applications

Purification of histidine-tagged and non-histidine-tagged recombinant and native proteins using IMAC chromatography.

The phosphate buffer may be used for other types of affinity chromatography methods.

Instructions For Use

Material Safety Data Sheet (MSDS)

Data Sheet/Technical Data

Certificate of Analysis (COA)

Other Documents