Lens culinaris lectin (LCA/LcH) (100 mg)

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Lens culinaris lectin (LCA/LcH) (100 mg)
Code: A05-0104-100mg
Manufacturer’s Part Number: 05-0104-100mg
Shipping Weight: 1.00 pounds
Quantity in Basket: None
Price: $717.00
Quantity:

Product Description

Lens culinaris lectin is isolated from lentil seeds by affinity chromatography. It has 2 subunits and a molecular weight of 46 kDa. The lectin gives two major bands in isoelectrofocusing corresponding to the isomers LCA-A and LCA-B.

Lens culinaris is not blood group specific. Its carbohydrate specificity is D-mannose and D-glucose. Adding Mn2+ and Ca2+ to the reconstitution buffer will enhance hemagglutination activity.

LCA contains a hemagglutinin A and B mixture that agglutinates human red blood cells but that is not group-specific. Lens culinaris agglutinates a 2% suspension of human erythrocytes at a lectin concentration of ≤6 µg/ml in 0.9% NaCl after 2 h at 25°C. Adding 60 mM methylmannoside gives an inhibition with a titer that is at least 16-fold lower than the control.

Lens culinaris lectin is supplied as a white lyophilized powder from a buffer containing 1 mM CaCl2, 1 mM MnCI2 and 1 mM MgCI2. No preservatives are added.

For laboratory use only.

Information

SKU ID# Packaging Website Links
A05-0104-100mg 100 mg www.buffer-solutions.com
 
Features Specifications Applications
Sugar specificity: α-D-mannose and α-D-glucose.
Hemagglutinating activity.
High activity.
Appearance: White lyophilized powder or flocculate.
Source: Lentil seeds.
Molecular weight: 46 kDa.
Sugar specificity: α-D-mannose and α-D-glucose.
Activity: Agglutinates human erythrocytes in a 2% blood suspension with lectin concentration ≤8 µg/ml in 0.9% NaCl after 2 h at 25°C.
Microorganisms: <100 CFU/g.
Protein content: >85%, OD280nm (ε1.6 = 1 mg/ml).
Identity: SDS-PAGE, three bands - the three isolectins.
Shelf life: >Three years when stored at -20°.
Hemagglutination.
Cell agglutination studies.

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Package Inserts MSDS Tech Specs Adaptations
Lens Culinaris Lens Culinaris N/A N/A