Lens Culinaris Lectin (LCA/LcH) (10 mg)
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Lens Culinaris Lectin (LCA/LcH) (10 mg)

Code: A05-0104-10
Manufacturer’s Part Number: 05-0104-10
Product size: 10 mg

Price: $111.00

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Product Description

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For Research Use Only

Lens culinaris lectin is isolated from lentil seeds by affinity chromatography. It has 2 subunits and a molecular weight of 46 kDa. The lectin gives two major bands in isoelectrofocusing corresponding to the isomers LCA-A and LCA-B.

Lens culinaris is not blood group specific. Its carbohydrate specificity is D-mannose and D-glucose. Adding Mn2+ and Ca2+ to the reconstitution buffer will enhance hemagglutination activity.

LCA contains a hemagglutinin A and B mixture that agglutinates human red blood cells but that is not group-specific. Lens culinaris agglutinates a 2% suspension of human erythrocytes at a lectin concentration of ≤ 6 µg/ml in 0.9% NaCl after 2 hrs at 25°C. Adding 60 mM methylmannoside gives an inhibition with a titer that is at least 16-fold lower than the control.

LCA/LcH is supplied as a white lyophilized powder from a buffer containing 1 mM CaCl2, 1 mM MnCI2 and 1 mM MgCI2. No preservatives are added.

For laboratory use only.

Product Characteristics

  • Sugar specificity: α-D-mannose and α-D-glucose.
  • Hemagglutinating activity.
  • High activity.

Product Components

  • Appearance: White lyophilized powder or flocculate.
  • Source: Lentil seeds.
  • Molecular weight: 46 kDa.
  • Sugar specificity: α-D-mannose and α-D-glucose.
  • Activity: Agglutinates human erythrocytes in a 2% blood suspension with lectin concentration ≤ 8 µg/ml in 0.9% NaCl after 2 hrs at 25°C.
  • Microorganisms: < 100 CFU/g.
  • Protein content: > 85%, OD280nm (ε 1.6 = 1 mg/ml).
  • Identity: SDS-PAGE, three bands - the three isolectins.
  • Shelf life: > Three years when stored at -20°.

Product Applications

Hemagglutination.

Cell agglutination studies.

Material Safety Data Sheet (MSDS)

Technical Data

Certificate of Analysis (COA)

Other Documents