5-KIT USP/EP-UFH Anti-IIa Heparin
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5-KIT USP/EP-UFH Anti-IIa Heparin

Code: A5D-50461
Manufacturer’s Part Number: 5D-50461
Product size: Kit
Clearance: RUO

Price: $593.00

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Product Description

This product is not available for purchase in North America and Nordic regions.

Complete kit for anti-IIa potency testing of Unfractionated Heparin (UFH) per both the USP (Chapter <208>) and European Pharmacopoeia (Chapter 2.7.12) methodologies. Chromogenic endpoint or kinetic assay measuring heparin anti-FIIa (anti-thrombin) activity in the range 0.005–0.030 USP Heparin Units/mL (IU/mL).

The anti-IIa method uses a constant amount of antithrombin (AT) incubated with sample heparin. Excess thrombin (Factor IIa) is added; residual thrombin cleaves a chromogenic thrombin substrate — colour intensity inversely proportional to heparin concentration, measured at 405 nm.

  • Dual pharmacopoeial compliance: USP Chapter <208> and Ph. Eur. Chapter 2.7.12
  • Range: 0.005–0.030 IU/mL anti-IIa
  • For UFH raw material and finished product potency research
  • Not available in North America and Nordic regions

Product Characteristics

Product Components

  • 1 pouch of A5D-80434, 5-BUFFER Tris-NaCI-EDTA-PEG 6000 Buffer salts pH 8.4
  • 3 x ~12 IU vials of Thrombin (Human)
  • 1 x 4 IU vial of Antithrombin (Human)
  • 1 x 12.5 mg vial of Chromogenic Thrombin Substrate

Product Applications

For Research Use Only. Not for Use in Diagnostic Procedures.

Complete kit for the measurement of heparin and heparin-like anticoagulants in buffer using an anti-FIIa chromogenic assay for pharmaceutical preparations in compliance with the EP and USP.

Material Safety Data Sheet (MSDS)

Data Sheet/Technical Data

Certificate of Analysis (COA)

Other Documents