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This Heparin Anti-FIIa method can be used as an endpoint or kinetic chromogenic assay for measuring the concentration of heparin and heparin-like anticoagulants in a concentration range of 0.005-0.030 USP Heparin Units/mL (IU/mL). The method is to be used for the anti-FIIa activity of Unfractionated Heparin following the recommendations of the European and US Pharmacopoeias.