Bacterial Strains

Xenometrix Ames tester strains are provided as individually packaged freeze-dried preparations with certified genotype verification and complete revival and maintenance protocols.

Each strain is verified for all required genetic markers prior to release: TA98 (hisD3052, rfa, uvrB, pKM101 plasmid --- frameshift, GC sites); TA100 (hisG46, rfa, uvrB, pKM101 plasmid --- base substitution, GC sites); TA1535 (hisG46, rfa, uvrB --- base substitution, no R-factor); TA1537 (hisC3076, rfa, uvrB --- frameshift, CG sites, no R-factor); E. coli WP2uvrA (trp, uvrA --- base substitution, AT sites). Strains are available individually or as complete five-strain OECD TG 471 sets.

Lot-specific spontaneous reversion frequency data, crystal violet sensitivity confirmation (rfa mutation), UV sensitivity confirmation (uvrB), and ampicillin resistance verification (pKM101) are included with each lot certificate.

Strains are stable for 12 months from manufacture when stored at minus 70 degrees C. Compatible with MacroAmes1 plate incorporation, Ames MPF 384-well, Ames II 48-well, and umuC SOS assay formats.